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KMID : 0382619880080020811
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Hanyang Journal of Medicine
1988 Volume.8 No. 2 p.811 ~ p.822
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Morphological Changes of an Acanthamoeba- species, YM-4 after Treatment with Antiserum
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Abstract
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Free-living amebas including Acanthamoeba species and Naegleria species may cause primary amebic meningo-encephalitis in human and experimental animals, and they are widely distributed in the natural circumstances. Acanthamoeba species, YM-4, isolated from gill of freshwater fish, Carassius carassius, was experimentally proved as a pathogenic strain for mice.
The present study was performed to evaluate the effect of antiserum on fine struc-ture of this ameba. Amebas were subcultured in casitone, glucose and vitamin (CGV) medium. For the experiment amebas were inoculated in mixed medium containing 2d% (v/v) of antiserum for 2 hours, and the observed with scanning and transmis-sion electron microscopes.
The results are summarized as follows:
1. The trophozoites projected many acanthopodia and the shape was irregular on SEM.
2. Antiserum-treated trophozoites showed round, immobilized ones without projec-tion of acanthopodium on SEM. Partial destruction of plasma membrane was often observed.
3. On TEM normal trophozoites were consisted of acanthopodia, large and round nucleus, food and contractile vacuoles, Golgi-complex, endoplasmic reticula, mitochondria, spongiomes around contractile vacuoles, and peroxisomes.
4. Antiserum-treated trophozoites showed partial destruction of plasma membrane, and projections of acanthopodium were rarely observed. In nucleus chromatin materials were aggregated, and on the wrinkled and irregular nuclear membrane ribosomal arrangement was increased. In the endoplasms decreased numbers of ribosomes, glycogen particles and lipid droplets were observed. Rough endoplasmic reticula were increased in distribution and often seen around mitochondria. Sometimes they surrounded mitochondria. Mitochondria were edematous and degenerated. Spongiome and peroxisome were could be not distinguished and not confirmed.
With above results it is presumed that specific antiserum destructs plasma mem-brane directly, and induces disturbances of ion exchanges and cell respiration foliw-ing immobilization and cell lysis.
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